Resources
WHO POLICY RECOMMENDATIONS & SUPPORTING DOCUMENTS
TRAINING PACKAGES
Training Package on XPERT MTB/RIF
Training Package on Acid-Fast Direct Smear Microscopy
Training Package on Culture in solid and liquid media - October 2012
(VERY LARGE zipped file 311 MB)
Training Package on DST by phenotypic and molecular methods - October 2012
(LARGE zipped file 34 MB)
Training Package on LPA (MTBDRplus, v.2)_- October 2012
(VERY LARGE zipped file 103 MB)
Training Package on Quality Management Systems (QMS)
- Laboratory Quality Management System Training Toolkit (English, French, Russian)
- The WHO/CDC/CLSI Laboratory Quality Management Handbook
The GLI Laboratory Toolbox
Download the complete Toolbox
(VERY LARGE zipped package 188 MB)
I. Contents
- A Roadmap for Laboratory Strengthening
Roadmap for Laboratory Strengthening - Standard Operating Procedures
Introduction guidance
List of SOPs
Module 1: Master SOP for writing quality management documents
Safety
Module 2: Procedure in case of spill of specimens in an AFB microscopy laboratory
Module 3: Procedure in case of spill of infectious material within the biological safety cabinets
Module 4: Emergency procedure in case of major biohazard incident outside the biological safety cabinet
Module 5: Fumigation of a biological safety cabinet
Module 6: Emergency procedure in case of fire
Module 7: Use of personal protective equipment in an AFB microscopy laboratory
Module 8: Use of personal protective equipment for culture and drug susceptibility testing
Module 9: Use of disinfectants
Use and maintenance of equipment
Module 10: Use and maintenance of class I and class II biological safety cabinets
Module 11: Use and maintenance of an autoclave
Module 12: Use and maintenance of an electromagnetic balance
Module 13: Use and maintenance of a centrifuge
Module 14: Use and maintenance of a freezer
Module 15: Use and maintenance of a refrigerator
Module 16: Use and maintenance of an incubator
Module 17: Use and maintenance of an inspissator
Module 18: Maintenance of a light microscope
Module 19: Maintenance of a fluorescence microscope
Module 20: Use and maintenance of a pH meter
Module 21: Use and maintenance of a water distiller
Preparation of reagents
Module 22: Preparation of reagents for microscopy
Module 23: Preparation of reagents for culture and drug-susceptibility testing
Module 24: Preparation of plain egg-based media
Module 25: Preparation of Löwenstein-Jensen drug-containing media
Procedures
Module 26: Ziehl-Neelsen staining
Module 27: Auramine staining
Module 28: Rechecking acid-fast bacilli smears for external quality assessment
Module 29: Sample conditions and transport for culture procedure
Module 30: Specimen processing for culture
Module 31: Drug susceptibility testing, proportion method
Module 32: Identification of Mycobacterium tuberculosis
Module 33: Niacin test
Module 34: Catalase test
Module 35: Nitrate reduction test
Module 36: Growth on PNB medium
Module 37: Maintenance of mycobacterial strains - Logistics Supply Management Tool
AFBsuppliesProcurementVs2
CU&DSTsupplies
Guidance for countries on the specifications for managing TB laboratory equipments and supplies
UserGuide_AFBlabSupplies
UserGuide_SuppliesCU&DSTworkbook - External Quality Assurance Package
AFB microscopy
EQA Overview & Planning
Facilitator Guide
Optional Modules
Panel Tests
Rechecking
Supervision - Management Information System
Culture & DST
French Versions
Microscopy & EQA - Culture and DST Package
Participant Manual
Trainer's Manual
II. Databases for Culture & DST