Resources


WHO POLICY RECOMMENDATIONS & SUPPORTING DOCUMENTS


TRAINING PACKAGES

Training Package on XPERT MTB/RIF

Training Package on Acid-Fast Direct Smear Microscopy

Training Package on Culture in solid and liquid media - October 2012
(VERY LARGE zipped file 311 MB)

Training Package on DST by phenotypic and molecular methods - October 2012
(LARGE zipped file 34 MB)

Training Package on LPA (MTBDRplus, v.2)_- October 2012
(VERY LARGE zipped file 103 MB)

Training Package on Quality Management Systems (QMS)

The GLI Laboratory Toolbox

Download the complete Toolbox
(VERY LARGE zipped package 188 MB)

I. Contents

  1. A Roadmap for Laboratory Strengthening
    Roadmap for Laboratory Strengthening

  2. Standard Operating Procedures
    Introduction guidance
    List of SOPs

    Module 1: Master SOP for writing quality management documents

    Safety
    Module 2: Procedure in case of spill of specimens in an AFB microscopy laboratory
    Module 3: Procedure in case of spill of infectious material within the biological safety cabinets
    Module 4: Emergency procedure in case of major biohazard incident outside the biological safety cabinet
    Module 5: Fumigation of a biological safety cabinet
    Module 6: Emergency procedure in case of fire
    Module 7: Use of personal protective equipment in an AFB microscopy laboratory
    Module 8: Use of personal protective equipment for culture and drug susceptibility testing
    Module 9: Use of disinfectants

    Use and maintenance of equipment
    Module 10: Use and maintenance of class I and class II biological safety cabinets
    Module 11: Use and maintenance of an autoclave
    Module 12: Use and maintenance of an electromagnetic balance
    Module 13: Use and maintenance of a centrifuge
    Module 14: Use and maintenance of a freezer
    Module 15: Use and maintenance of a refrigerator
    Module 16: Use and maintenance of an incubator
    Module 17: Use and maintenance of an inspissator
    Module 18: Maintenance of a light microscope
    Module 19: Maintenance of a fluorescence microscope
    Module 20: Use and maintenance of a pH meter
    Module 21: Use and maintenance of a water distiller

    Preparation of reagents
    Module 22: Preparation of reagents for microscopy
    Module 23: Preparation of reagents for culture and drug-susceptibility testing
    Module 24: Preparation of plain egg-based media
    Module 25: Preparation of Löwenstein-Jensen drug-containing media

    Procedures
    Module 26: Ziehl-Neelsen staining
    Module 27: Auramine staining
    Module 28: Rechecking acid-fast bacilli smears for external quality assessment
    Module 29: Sample conditions and transport for culture procedure
    Module 30: Specimen processing for culture
    Module 31: Drug susceptibility testing, proportion method
    Module 32: Identification of Mycobacterium tuberculosis
    Module 33: Niacin test
    Module 34: Catalase test
    Module 35: Nitrate reduction test
    Module 36: Growth on PNB medium
    Module 37: Maintenance of mycobacterial strains

  3. Logistics Supply Management Tool
    AFBsuppliesProcurementVs2
    CU&DSTsupplies
    Guidance for countries on the specifications for managing TB laboratory equipments and supplies
    UserGuide_AFBlabSupplies
    UserGuide_SuppliesCU&DSTworkbook

  4. External Quality Assurance Package
    AFB microscopy
    EQA Overview & Planning
    Facilitator Guide
    Optional Modules
    Panel Tests
    Rechecking
    Supervision

  5. Management Information System
    Culture & DST
    French Versions
    Microscopy & EQA

  6. Culture and DST Package
    Participant Manual
    Trainer's Manual

II. Databases for Culture & DST

MANUALS, GUIDELINES & TOOLS


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